Agrobacterium-mediated Genetic Transformation in Lentil (Lens culinaris Medik.) followed by In vitro Flowering and Seed Formation
نویسندگان
چکیده
منابع مشابه
Indirect in vitro regeneration of lentil (Lens culinaris Medik.)
Establishment of an efficient and reproducible regeneration protocol is one of the basic prerequisites for genetic transformation of any crop plant. In vitro culture of lentil has proven to be difficult. In spite of a number of reports on the regeneration of this plant, very few satisfying and reproducible protocol has yet been reported. This study carried out for investigation of diff...
متن کاملDirect in vitro regeneration of lentil (Lens culinaris Medik.).
This study surveyed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting by different explants and different concentration of BAP. Due to optimization of shoot regeneration, two media including of MS and modified MS (MS salts with double concentration of CaC21, and B5 vitamins), different explants such as decapitated embryo axes, epicotyls and cotyledonary no...
متن کاملWill selenium increase lentil (Lens culinaris Medik) yield and seed quality?
Lentil (Lens culinaris Medik), a nutritious traditional pulse crop, has been experiencing a declining area of production in South East Asia, due to lower yields, and marginal soils. The objective of this study was to determine whether selenium (Se) fertilization can increase lentil yield, productivity, and seed quality (both seed Se concentration and speciation). Selenium was provided to five l...
متن کاملEstimation of Genetic Diversity in Six Lentil (Lens culinaris Medik.) Varieties using Morphological and Biochemical markers
Morphological characters and Electrophoretic SDS-PAGE analysis were performed to established genetic diversity for six important lentil varieties of Bangladesh and to elucidate their genetic relationships. Analyses of variance of morphological characters showed significant differences among varieties. The resulted protein banding pattern showed 57.12% polymorphism and could be considered as gen...
متن کاملindirect in vitro regeneration of lentil (lens culinaris medik.)
establishment of an efficient and reproducible regeneration protocol is one of the basic prerequisites for genetic transformation of any crop plant. in vitro culture of lentil has proven to be difficult. in spite of a number of reports on the regeneration of this plant, very few satisfying and reproducible protocol has yet been reported. this study carried out for investigation of different hor...
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ژورنال
عنوان ژورنال: Plant Tissue Culture and Biotechnology
سال: 2012
ISSN: 1818-8745,1817-3721
DOI: 10.3329/ptcb.v22i1.11243